References:
1. Skoog, West, Holler and Crouch,
Analytical Chemistry: An Introduction, pages 626 to 627.
2. Skoog, Holler, and Nieman, Principles
of Instrumental Analysis, Chapters 8 and 9, pages 192 to 227,
3. Slides on Atomic Absorption Spectroscopy
by Varian (available from Dr. Moore)
4. Professor Moore's handouts on calibration
curves, standard addition and internal standards
Websites:
Flame photometry and Atomic Absorption Spectroscopy
http://www.science.uts.edu.au/subjects/91326/Section7/
Atomic Absorption Spectroscopy tutorial
http://www.chem.vt.edu/chem-ed/ac-meths.htmlAtomic Absorption Spectroscopy tutorial
http://www.people.virginia.edu/~roa2s/chem_551/4/Determination of iron in Foods by Atomic Absorption Spectroscopy
http://quantlab.bu.edu/quantlab/2000-Spring/exp4.htmlExperiment on Flame Photometry
http://www.chem.usu.edu/faculty/SBialkow/Classes/361/flame.htmlShockwave demo=s on continuous and line spectra
http://phys.educ.ksu.edu/vqm/html/emission.html
http://phys.educ.ksu.edu/vqm/html/absorption.html
1. Be familiar with the different spectra exhibited by flame photometry and by atomic absorption spectroscopy and the basis (cause) of this different spectra. (Hint: explain in terms of energy levels)
2. Give the similarities and differences in comparison of components in a flame photometer and an atomic absorption spectrophotometer. (Remember that big chart I asked you to develop as you study different instruments!) Radiation source Sample Monochromator Slits Detector
3. Discuss the makeup of hollow cathode lamp (HCL) and electrodeless discharge lamps (EDL).
4. What is the purpose of a nebulizer/atomizer?
5. Explain the role of flame and choice of fuel mixtures in producing spectra.
6. Identify types of monochromators in a flame photometer and in an atomic absorption spectrophotometer.
7. Explain the difference and advantages/disadvantages of a single beam vs a double beam spectrophotometer.
8. Why is a background corrector necessary for some analytical procedures using AAS? How is usually done?
9. Explain spectral, chemical, and matrix interferences in an atomic absorption analysis.
10. Explain the different methods of analysis: calibration curves, standard addition and internal standard and be able to do the necessary math work and apply them to problem solving.
11.What are detection, signal/noise and sensitivity levels? How can these be determined experimentally?